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基因敲除技术是建立在同源重组技术之上,可对基因组进行定位修饰的实验方法。本文简述了基因敲除技术的基本原理、打靶策略、筛选机制,在动植物和微生物中常用的基因敲除方法以及基因敲除的应用。
Abstract：Gene knockout was built on homologous recombination,and might be positional modified on the genome.This paper introduced the principle,the targeting strategies,the screening mechanism and the commonly method in animals,plants and micro-organisms of gene knockout.The application of gene knockout was also prospected in this paper.
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中图分类号:Q78
引用信息:
[1]黄敏.基因敲除技术及其应用[J].广东轻工职业技术学院学报,2009,8(04):11-14.
2009-12-15
2009-12-15